Molecular Cloning: A Laboratory Manual, Book 3 |
From inside the book
Results 1-3 of 96
Page 172
... proteins . These antibodies have been used ( 1 ) to purify proteins by immunoaffinity chromatography ; ( 2 ) in diagnostic assays to quantitate the levels of protein ; and ( 3 ) to localize the proteins in organisms , tissues , and ...
... proteins . These antibodies have been used ( 1 ) to purify proteins by immunoaffinity chromatography ; ( 2 ) in diagnostic assays to quantitate the levels of protein ; and ( 3 ) to localize the proteins in organisms , tissues , and ...
Page 17-2
... proteins . These antibodies have been used ( 1 ) to purify proteins by immunoaffinity chromatography ; ( 2 ) in diagnostic assays to quantitate the levels of protein ; and ( 3 ) to localize the proteins in organisms , tissues , and ...
... proteins . These antibodies have been used ( 1 ) to purify proteins by immunoaffinity chromatography ; ( 2 ) in diagnostic assays to quantitate the levels of protein ; and ( 3 ) to localize the proteins in organisms , tissues , and ...
Page 17-29
... protein not have an amino - terminal methionine , which is coded for by the ATG in these vectors . The amino ... Protein That Can Be Cleaved by a Protease or Cyanogen Bromide The cloned gene can be expressed as part of a fusion protein ...
... protein not have an amino - terminal methionine , which is coded for by the ATG in these vectors . The amino ... Protein That Can Be Cleaved by a Protease or Cyanogen Bromide The cloned gene can be expressed as part of a fusion protein ...
Other editions - View all
Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml