Molecular Cloning: A Laboratory Manual, Book 3 |
From inside the book
Results 1-3 of 41
Page 1661
... radioactive ink to align the plate with the film , and then expose the plate to X - ray film . Do not cover the TLC plate with Saran Wrap , which will block the relatively weak radiation emitted by 14C . For preparation of radioactive ...
... radioactive ink to align the plate with the film , and then expose the plate to X - ray film . Do not cover the TLC plate with Saran Wrap , which will block the relatively weak radiation emitted by 14C . For preparation of radioactive ...
Page 18-28
... radioactive medium in the radioactive waste . If the target antigen is a secreted protein , save the medium for immunoprecipitation ( see pages 18.44-18.46 ) . 5. Transfer the petri dish to a flat surface chilled to 0 ° C ( e.g. , an ...
... radioactive medium in the radioactive waste . If the target antigen is a secreted protein , save the medium for immunoprecipitation ( see pages 18.44-18.46 ) . 5. Transfer the petri dish to a flat surface chilled to 0 ° C ( e.g. , an ...
Page 18-73
... radioactive ink to adhesive labels or tape , which are then attached to the surface of the 3MM paper . Radioactive ink can be made by adding a small amount of Tran35S - label ( ICN Radiochemicals ) or 32P to a microfuge tube containing ...
... radioactive ink to adhesive labels or tape , which are then attached to the surface of the 3MM paper . Radioactive ink can be made by adding a small amount of Tran35S - label ( ICN Radiochemicals ) or 32P to a microfuge tube containing ...
Other editions - View all
Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml