Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1584
... RADIOLABELED PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 ...
... RADIOLABELED PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 ...
Page 18-21
... radiolabeled antibody . Antibodies radiolabeled with 125I as described later in this chapter ( see pages 18.24- 18.25 ) have an approximate specific activity of 5 × 106 cpm / μg . The amount of the radiolabeled second antibody that ...
... radiolabeled antibody . Antibodies radiolabeled with 125I as described later in this chapter ( see pages 18.24- 18.25 ) have an approximate specific activity of 5 × 106 cpm / μg . The amount of the radiolabeled second antibody that ...
Page 18-26
... radiolabeled protein . When coupled with SDS - polyacrylamide gel electrophoresis , the technique is ideal for analysis of the synthesis and processing of foreign antigens expressed in prokaryotic and eukaryotic hosts or in in vitro ...
... radiolabeled protein . When coupled with SDS - polyacrylamide gel electrophoresis , the technique is ideal for analysis of the synthesis and processing of foreign antigens expressed in prokaryotic and eukaryotic hosts or in in vitro ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml