Molecular Cloning: A Laboratory Manual, Book 3 |
From inside the book
Results 1-3 of 89
Page 1659
... Remove the medium from cells growing in monolayers in 90 - mm tissue culture dishes by gentle aspiration . Wash the monolayers three times with 5 ml of phosphate - buffered saline ( PBS ; see Appendix B ) lacking magnesium and calcium ...
... Remove the medium from cells growing in monolayers in 90 - mm tissue culture dishes by gentle aspiration . Wash the monolayers three times with 5 ml of phosphate - buffered saline ( PBS ; see Appendix B ) lacking magnesium and calcium ...
Page 18-14
... remove antibacterial antibodies from antisera used for such screening are described on pages 12.25-12.28 . In the section that follows , we describe techniques to remove antibodies that cross - react with components in eukaryotic cells ...
... remove antibacterial antibodies from antisera used for such screening are described on pages 12.25-12.28 . In the section that follows , we describe techniques to remove antibodies that cross - react with components in eukaryotic cells ...
Page 18-15
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Removal of Cross - reacting Antibodies from Antisera To remove antibodies that react with antigens present in mammalian cells , use an acetone extract of a cell line or tissue that is ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Removal of Cross - reacting Antibodies from Antisera To remove antibodies that react with antigens present in mammalian cells , use an acetone extract of a cell line or tissue that is ...
Other editions - View all
Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml