Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-63
... sample in a boiling - water bath for 10 minutes . The sample should become highly viscous as a consequence of the release of high- molecular - weight chromosomal DNA . 3. Shear the chromosomal DNA by sonication , using either a ...
... sample in a boiling - water bath for 10 minutes . The sample should become highly viscous as a consequence of the release of high- molecular - weight chromosomal DNA . 3. Shear the chromosomal DNA by sonication , using either a ...
Page E-5
... sample is pure ( i.e. , without significant amounts of contaminants such as proteins , phenol , agarose , or other nucleic acids ) , spectrophotometric measurement of the amount of ultraviolet irradiation absorbed by the bases is simple ...
... sample is pure ( i.e. , without significant amounts of contaminants such as proteins , phenol , agarose , or other nucleic acids ) , spectrophotometric measurement of the amount of ultraviolet irradiation absorbed by the bases is simple ...
Page E-6
... sample with those of the standard solutions . AGAROSE PLATE METHOD Contaminants that may be present in the DNA sample can either contribute to or quench the fluorescence . To avoid these problems , the DNA samples and standards can be ...
... sample with those of the standard solutions . AGAROSE PLATE METHOD Contaminants that may be present in the DNA sample can either contribute to or quench the fluorescence . To avoid these problems , the DNA samples and standards can be ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml