Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1643
... serum - free medium . Add to the cells 5 ml ( per 60 - mm dish ) or 3 ml ( per 35 - mm dish ) of medium supplemented with serum , and incubate the cultures for 36-60 hours at 37 ° C in a humidified incubator in an atmosphere of 5-7 ...
... serum - free medium . Add to the cells 5 ml ( per 60 - mm dish ) or 3 ml ( per 35 - mm dish ) of medium supplemented with serum , and incubate the cultures for 36-60 hours at 37 ° C in a humidified incubator in an atmosphere of 5-7 ...
Page 1647
... serum - containing alpha medium . Remove the DMSO solution by aspiration , wash the cells once with prewarmed ( 37 ° C ) , serum - free medium , and add 10 ml of complete medium containing 10 % fetal calf serum . Incubate the cultures ...
... serum - containing alpha medium . Remove the DMSO solution by aspiration , wash the cells once with prewarmed ( 37 ° C ) , serum - free medium , and add 10 ml of complete medium containing 10 % fetal calf serum . Incubate the cultures ...
Page 18-24
... serum albumin through the column . Then wash the column with 5 volumes of PBS lacking bovine serum albumin . When the column is ready , wrap a piece of Parafilm around the bottom of the pipette . This prevents the column from running ...
... serum albumin through the column . Then wash the column with 5 volumes of PBS lacking bovine serum albumin . When the column is ready , wrap a piece of Parafilm around the bottom of the pipette . This prevents the column from running ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml