Molecular Cloning: A Laboratory Manual, Book 3 |
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Page A-2
... solution of 0.17 м KH2PO4 , 0.72 м K2HPO4 . ( This solution is made by dissolving 2.31 g of KH , PO , and 12.54 g of K2HPO , in 90 ml of deionized H2O . After the salts have dissolved , adjust the volume of the solution to 100 ml with ...
... solution of 0.17 м KH2PO4 , 0.72 м K2HPO4 . ( This solution is made by dissolving 2.31 g of KH , PO , and 12.54 g of K2HPO , in 90 ml of deionized H2O . After the salts have dissolved , adjust the volume of the solution to 100 ml with ...
Page B-9
A Laboratory Manual Joseph Sambrook, Tom Maniatis. STOCK SOLUTIONS AND BLOCKING AGENTS TABLE B.7 Preparation of Commonly Used Stock Solutions Solution Method of preparation Comments TABLE B.7 ( continued ) Solution Method of preparation ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. STOCK SOLUTIONS AND BLOCKING AGENTS TABLE B.7 Preparation of Commonly Used Stock Solutions Solution Method of preparation Comments TABLE B.7 ( continued ) Solution Method of preparation ...
Page E-8
... solution , add 0.2 volume of fresh 5 % hypophosphorous acid and 0.12 volume of fresh 0.5 M sodium nitrite . Mix carefully . Important : Check that the pH of the solution is < 3.0 . Hypophosphorous acid is usually supplied as a 50 % solution ...
... solution , add 0.2 volume of fresh 5 % hypophosphorous acid and 0.12 volume of fresh 0.5 M sodium nitrite . Mix carefully . Important : Check that the pH of the solution is < 3.0 . Hypophosphorous acid is usually supplied as a 50 % solution ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml