Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 24
Page A-3
... sterilized by autoclaving for 15 minutes at 15 lb / sq . in . on liquid cycle . The MgSO , and CaCl , solutions should be prepared separately , sterilized by autoclaving , and added after diluting the 5 × M9 salts to 1 liter with sterile ...
... sterilized by autoclaving for 15 minutes at 15 lb / sq . in . on liquid cycle . The MgSO , and CaCl , solutions should be prepared separately , sterilized by autoclaving , and added after diluting the 5 × M9 salts to 1 liter with sterile ...
Page A-5
... sterile glycerol ( sterilized by autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle ) . Vortex the culture to ensure that the glycerol is evenly dispersed . Transfer the culture to a labeled storage tube equipped with a ...
... sterile glycerol ( sterilized by autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle ) . Vortex the culture to ensure that the glycerol is evenly dispersed . Transfer the culture to a labeled storage tube equipped with a ...
Page A-7
... sterile 20 % maltose solution for every 100 ml of medium . Make up a sterile 20 % stock solution of maltose as follows : maltose H2O to 100 ml 20 g Sterilize the solution by filtration through a 0.22 - micron filter . Store the sterile ...
... sterile 20 % maltose solution for every 100 ml of medium . Make up a sterile 20 % stock solution of maltose as follows : maltose H2O to 100 ml 20 g Sterilize the solution by filtration through a 0.22 - micron filter . Store the sterile ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml