Molecular Cloning: A Laboratory Manual, Book 3 |
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Page A-9
A Laboratory Manual Joseph Sambrook, Tom Maniatis. BACTERIAL STRAIN LIST TABLE A.2 Bacterial Strains Strain 71/18 BB4 BHB2688 BHB2690 BL21 ( DE3 ) Genotype supE thi △ ( lac - proAB ) + F ' [ proAB lacl lacZAM15 ] supF58 supE44 hsdR514 ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. BACTERIAL STRAIN LIST TABLE A.2 Bacterial Strains Strain 71/18 BB4 BHB2688 BHB2690 BL21 ( DE3 ) Genotype supE thi △ ( lac - proAB ) + F ' [ proAB lacl lacZAM15 ] supF58 supE44 hsdR514 ...
Page A-10
... strain that will support growth of vectors carrying amber mutations and will modify but not restrict transfected DNA ( Yanisch - Perron et al . 1985 ) . A suppressing strain used to propagate bacteriophage vectors and their recombinants ...
... strain that will support growth of vectors carrying amber mutations and will modify but not restrict transfected DNA ( Yanisch - Perron et al . 1985 ) . A suppressing strain used to propagate bacteriophage vectors and their recombinants ...
Page A-11
... strain used as a host for plasmids containing the bacteriophage △ PL promoter ( Nagai and Thøgersen 1984 ) . λ + A recA derivative of HB101 that can be transformed with high efficiency ( Bolivar et al . 1977 ; Peacock et al . 1981 ; B ...
... strain used as a host for plasmids containing the bacteriophage △ PL promoter ( Nagai and Thøgersen 1984 ) . λ + A recA derivative of HB101 that can be transformed with high efficiency ( Bolivar et al . 1977 ; Peacock et al . 1981 ; B ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml