Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-19
... target antigens . These assays include immunoprecipitation , western ... antigen . This means that RIAS are sufficiently sensitive to measure , for ... target protein in the test sample competes with a constant amount of radiolabeled ...
... target antigens . These assays include immunoprecipitation , western ... antigen . This means that RIAS are sufficiently sensitive to measure , for ... target protein in the test sample competes with a constant amount of radiolabeled ...
Page 18-42
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Formation of Antigen - Antibody Complexes In this procedure , specific antibody directed against the target protein is added to aliquots of cell lysates . Antigen - antibody complexes ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Formation of Antigen - Antibody Complexes In this procedure , specific antibody directed against the target protein is added to aliquots of cell lysates . Antigen - antibody complexes ...
Page 18-60
... antibodies that react specifically with an- tigenic epitopes displayed by the target protein attached to the solid support . Western blotting is therefore extremely useful for the identification and quantitation of specific proteins in ...
... antibodies that react specifically with an- tigenic epitopes displayed by the target protein attached to the solid support . Western blotting is therefore extremely useful for the identification and quantitation of specific proteins in ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml