Molecular cloning: a laboratory manual, Volume 3 |
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User Review - iayork - LibraryThingthe BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review
LibraryThing Review
User Review - iayork - LibraryThingthe BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review
Contents
Cosmid Vectors | 16-3 |
Hybridization 11 | 16-11 |
PREPARATION OF WORKING SOLUTIONS OF dNTPs 13 60 | 16-13 |
Copyright | |
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Common terms and phrases
Acad acetate acrylamide activity aliquots amount antigen antisera aqueous assay bacteria bacteriophage T4 binding Biol cDNA cell lines chloramphenicol Cl pH cloned gene codon column containing culture denatured dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract film foreign protein fragment hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture monoclonal antibodies mRNA NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides P.O. Box pellet phosphate plasmid plate PMSF polyclonal precipitate prepared Proc promoter protein of interest radioactive radiolabeled reaction reagent remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sodium azide staining sterile stock solution stored supernatant target antigen target protein Telephone termini transcription transfection transfer transient expression volume wash western blotting