Molecular Cloning: A Laboratory Manual, Book 3

acetate acrylamide activity Adjust the volume aliquots amount antibody antigen antisera aqueous assay autoclaving bacteria bacteriophage T4 Biol bromophenol blue cDNA cell lines Cl pH cloned gene codon coli column containing culture deionized H2O dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract foreign protein fragment fusion protein HindIII hybridization immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNA NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides P.O. Box pag/ml pellet phosphate plasmid plate PMSF polycloning precipitate prepared promoter radioactive radiolabeled reaction recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfected transfer transformation Tris wash western blotting