Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1604
... transfected efficiently and not all of them will necessarily carry out exactly the same set of posttranslational modifica- tions . For example , Chinese hamster ovary ( CHO ) cells typically add more terminal sialic acid residues to ...
... transfected efficiently and not all of them will necessarily carry out exactly the same set of posttranslational modifica- tions . For example , Chinese hamster ovary ( CHO ) cells typically add more terminal sialic acid residues to ...
Page 1617
... transfected into cultured mammalian cells . Because these vectors do not contain a eukaryotic replicon , no episomal amplification of the transfected DNA occurs . Instead , the transfected DNA integrates into the genomes of a minority ...
... transfected into cultured mammalian cells . Because these vectors do not contain a eukaryotic replicon , no episomal amplification of the transfected DNA occurs . Instead , the transfected DNA integrates into the genomes of a minority ...
Page 1658
... transfected mammalian cells from a promoter with a broad host range ( the SV40 early promoter or the Rous sarcoma virus LTR ) . Extracts of most types of mammalian cells contain relatively low levels of endogenous ẞ - galactosi- dase ...
... transfected mammalian cells from a promoter with a broad host range ( the SV40 early promoter or the Rous sarcoma virus LTR ) . Extracts of most types of mammalian cells contain relatively low levels of endogenous ẞ - galactosi- dase ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml