Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1592
... Transfection of Cells Growing in Suspension 16.38 Modified Calcium Phosphate - mediated Transfection Procedure 16.39 TRANSFECTION MEDIATED BY DEAE - DEXTRAN 16.41 Transfection Using DEAE - Dextran : Protocol I 16.42 Transfection Using ...
... Transfection of Cells Growing in Suspension 16.38 Modified Calcium Phosphate - mediated Transfection Procedure 16.39 TRANSFECTION MEDIATED BY DEAE - DEXTRAN 16.41 Transfection Using DEAE - Dextran : Protocol I 16.42 Transfection Using ...
Page 1641
... transfection of viral genomes and plasmids carrying viral sequences . Although the mechanism of action of DEAE - dextran is not known , it is thought that the polymer might bind to DNA and inhibit the action of nucleases and / or bind ...
... transfection of viral genomes and plasmids carrying viral sequences . Although the mechanism of action of DEAE - dextran is not known , it is thought that the polymer might bind to DNA and inhibit the action of nucleases and / or bind ...
Page 1642
... transfection was > 2 × 10 ° ( McCutchan and Pagano 1968 ) . Although this material is no longer available commercially , it is still occasionally found in chemical storerooms . The older batches of higher - molecular - weight DEAE ...
... transfection was > 2 × 10 ° ( McCutchan and Pagano 1968 ) . Although this material is no longer available commercially , it is still occasionally found in chemical storerooms . The older batches of higher - molecular - weight DEAE ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml