Molecular Cloning: A Laboratory Manual, Book 3 |
From inside the book
Results 1-3 of 93
Page 1584
... TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS 9.34 Transfer of DNA to Nitrocellulose Filters 9.38 CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 9.38 SIMULTANEOUS TRANSFER OF DNA FROM A SINGLE AGAROSE GEL TO TWO ...
... TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS 9.34 Transfer of DNA to Nitrocellulose Filters 9.38 CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 9.38 SIMULTANEOUS TRANSFER OF DNA FROM A SINGLE AGAROSE GEL TO TWO ...
Page 1663
... Transfer the sample to an ice bath , and add 100 μl of ice - cold ethyl acetate . Mix the phases by vigorous vortexing . Centrifuge the mixtures at 12,000g for 3 minutes at room temperature in a microfuge . 4. Transfer 80 μl of the ...
... Transfer the sample to an ice bath , and add 100 μl of ice - cold ethyl acetate . Mix the phases by vigorous vortexing . Centrifuge the mixtures at 12,000g for 3 minutes at room temperature in a microfuge . 4. Transfer 80 μl of the ...
Page 18-64
... transfer . Furthermore , charged supports such as nylon membranes may not bind proteins of the same charge with high efficiency . Consequently , most western blotting nowadays is carried out by direct electrophoretic transfer of ...
... transfer . Furthermore , charged supports such as nylon membranes may not bind proteins of the same charge with high efficiency . Consequently , most western blotting nowadays is carried out by direct electrophoretic transfer of ...
Other editions - View all
Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml