Molecular Cloning: A Laboratory Manual, Book 3 |
From inside the book
Results 1-3 of 64
Page 1646
... transformation ) . 7. a . Transient expression : Harvest the cells 48-60 hours after transfection for analysis of RNA or DNA by hybridization . Newly synthesized protein may be analyzed by radioimmunoassay , by western blotting , by ...
... transformation ) . 7. a . Transient expression : Harvest the cells 48-60 hours after transfection for analysis of RNA or DNA by hybridization . Newly synthesized protein may be analyzed by radioimmunoassay , by western blotting , by ...
Page 1647
... transformation of CHO cells by plasmid DNA , yielding approximately 15 - fold more trans- formants than calcium phosphate - DNA coprecipitation . However , there is no difference between the two methods in the efficiency of transformation ...
... transformation of CHO cells by plasmid DNA , yielding approximately 15 - fold more trans- formants than calcium phosphate - DNA coprecipitation . However , there is no difference between the two methods in the efficiency of transformation ...
Page 1652
... transformation will almost certainly be very low . 3. Centrifuge the mixture of bacterial protoplasts and mammalian cells at 2000 rpm for 10 minutes at room temperature in a Beckman TJ6 cen- trifuge ( or its equivalent ) . Remove the ...
... transformation will almost certainly be very low . 3. Centrifuge the mixture of bacterial protoplasts and mammalian cells at 2000 rpm for 10 minutes at room temperature in a Beckman TJ6 cen- trifuge ( or its equivalent ) . Remove the ...
Other editions - View all
Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml