Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-31
... μg / ml trypsin Stock solution 10 mg / ml in 0.01 M HEPES ( pH 8.0 ) chymotrypsin plasmin Leupeptins plasmin ( supplied as a 3 : 1 trypsin chymotrypsin pepsin 1-2 μg / ml 10 mg / ml in water mixture of propionyl papain cathepsins A and ...
... μg / ml trypsin Stock solution 10 mg / ml in 0.01 M HEPES ( pH 8.0 ) chymotrypsin plasmin Leupeptins plasmin ( supplied as a 3 : 1 trypsin chymotrypsin pepsin 1-2 μg / ml 10 mg / ml in water mixture of propionyl papain cathepsins A and ...
Page 18-32
... μg / ml phenylmethylsulfonyl fluoride ( PMSF ) 1 μg / ml aprotinin 1 % Nonidet P - 40 ( NP - 40 ) 0.5 % sodium deoxycholate Single - detergent lysis buffer 50 mM Tris Cl ( pH 8.0 ) 150 mM NaCl 0.02 % sodium azide 100 μg / ml PMSF 1 μg ...
... μg / ml phenylmethylsulfonyl fluoride ( PMSF ) 1 μg / ml aprotinin 1 % Nonidet P - 40 ( NP - 40 ) 0.5 % sodium deoxycholate Single - detergent lysis buffer 50 mM Tris Cl ( pH 8.0 ) 150 mM NaCl 0.02 % sodium azide 100 μg / ml PMSF 1 μg ...
Page A-6
... μg / ml 60 μg / ml Carbenicillin 50 mg / ml in H. , O -20 ° C 20 μg / ml 60 μg / ml Chloramphenicol 34 mg / ml in ethanol -20 ° C 25 μg / ml 170 μg / ml Kanamycin Streptomycin 10 mg / ml in H2O -20 ° C 10 μg / ml 50 μg / ml 10 mg / ml ...
... μg / ml 60 μg / ml Carbenicillin 50 mg / ml in H. , O -20 ° C 20 μg / ml 60 μg / ml Chloramphenicol 34 mg / ml in ethanol -20 ° C 25 μg / ml 170 μg / ml Kanamycin Streptomycin 10 mg / ml in H2O -20 ° C 10 μg / ml 50 μg / ml 10 mg / ml ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml