Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1638
... wash them once with PBS . Resuspend the cells in 10 ml of prewarmed complete growth medium . Return the cells to the incubator for 48 hours before assaying for transient expression of transfected genes ( step 6a , page 16.36 ) or ...
... wash them once with PBS . Resuspend the cells in 10 ml of prewarmed complete growth medium . Return the cells to the incubator for 48 hours before assaying for transient expression of transfected genes ( step 6a , page 16.36 ) or ...
Page 18-45
... wash protein A - antigen - antibody complexes . The aim is to displace proteins that are nonspecifically adsorbed to ... wash is carried out with NET - gel buffer supplemented with NaCl to a final concentration of 0.5 M. For the second ...
... wash protein A - antigen - antibody complexes . The aim is to displace proteins that are nonspecifically adsorbed to ... wash is carried out with NET - gel buffer supplemented with NaCl to a final concentration of 0.5 M. For the second ...
Page 18-72
... Wash the filter in several changes of PBS ( 10 minutes each change ) . Continue washing until no radioactivity is detected by a hand - held minimonitor in regions of the filter that carry no protein . 5. Remove the filter from the last wash ...
... Wash the filter in several changes of PBS ( 10 minutes each change ) . Continue washing until no radioactivity is detected by a hand - held minimonitor in regions of the filter that carry no protein . 5. Remove the filter from the last wash ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml