Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-60
... ( WESTERN BLOTTING ) Western blotting ( Towbin et al . 1979 ; Burnette 1981 ) is to proteins what Southern blotting is to DNA . In both techniques , electrophoretically sepa- rated components are transferred from a gel to a solid support ...
... ( WESTERN BLOTTING ) Western blotting ( Towbin et al . 1979 ; Burnette 1981 ) is to proteins what Southern blotting is to DNA . In both techniques , electrophoretically sepa- rated components are transferred from a gel to a solid support ...
Page 18-63
... western blotting . The lowest amount of an average - sized protein that can be detected by western blotting is approximately 1-5 ng . Approximately 100 μg of total cellular protein can be applied to a lane in a 0.75 - mm - thick SDS ...
... western blotting . The lowest amount of an average - sized protein that can be detected by western blotting is approximately 1-5 ng . Approximately 100 μg of total cellular protein can be applied to a lane in a 0.75 - mm - thick SDS ...
Page 18-64
... western blotting nowadays is carried out by direct electrophoretic transfer of proteins from the gel to a nitrocellulose filter ( Burnette 1981 ) . Two types of electrophoresis apparatuses are available for electroblotting . In the ...
... western blotting nowadays is carried out by direct electrophoretic transfer of proteins from the gel to a nitrocellulose filter ( Burnette 1981 ) . Two types of electrophoresis apparatuses are available for electroblotting . In the ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml