Molecular Cloning: A Laboratory Manual, Book 3 |
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acetate acrylamide Adjust the volume agarose aliquots ammonium persulfate amount antibody antigen assay autoclaving bacterial bromophenol blue cDNA centrifugation cloned coli column containing culture deionized H₂O denatured dilution Dissolve dithiothreitol DNA polymerase dNTPs EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract film final concentration fragment gel electrophoresis gene H H OH hsdR hybridization Incubate lacZAM15 lb/sq ligation linkers liquid cycle lysate mammalian cells medium method mg/ml MgCl2 microfuge tube minutes at 15 minutes at 4°C mixture ml of H₂O Molecular mRNAs NaCl nitrocellulose nitrocellulose filter nucleic acid OH OH P.O. Box pellet phenol phosphate plasmid plates PMSF precipitation prepared protein radioactive radiolabeled reaction reagent remove restriction enzyme RNAase room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution supernatant target protein Telephone termini transfected transfer Tris Cl pH vectors wash western blotting µg/ml