PCR protocols: a guide to methods and applicationsThe correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual. Key Features * This first-rate guide will help you * Avoid contamination--with specific instructions on setting up your lab * Avoid cumbersome molecular biological techniques * Discover new applications * Simply call our toll-free, 800 number listed below or cut out the coupon, fill in the blanks, and mail it to us with your check, credit-card number, or money order; We'll send you the book and you'll get your answers |
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Contents
Amplification of Genomic DNA | 13 |
Rapid Amplification of cDNA Ends | 28 |
cDNA Cloning Using Degenerate Primers | 46 |
Copyright | |
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Common terms and phrases
1990 by Academic Academic Press agarose gel aliquots alleles amplification products amplified DNA analysis annealing Applications Copyright assay cDNA cells chromosome cloning concentration containing cycles denaturation detection digestion DNA fragments DNA sequences dNTP dot blot EDTA enzymatic amplification ethanol ethidium bromide extraction Faloona form reserved gene genetic genomic genomic DNA Guide to Methods H. A. Erlich Higuchi hybridization incubated labeled lanes Literature Cited membrane Methods and Applications MgCl2 minutes molecular molecules mRNA mutations Natl Nucleic Acids Res nucleotide oligomer oligonucleotide probes PCR amplification PCR primers PCR product PCR Protocols Perkin-Elmer Cetus phage pmol polymerase chain reaction polymorphisms prepared primer primer pair procedure purified quences reagents recombinant region restriction enzymes reverse transcriptase rights of reproduction rRNA Saiki Scharf single-stranded Sninsky solution Southern blot specific SSPE strand synthesis Taq DNA polymerase Taq polymerase temperature template tion tissue transcription Tris-HCl pH tube virus vitro
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Bibliographic information
| Title | PCR protocols: a guide to methods and applications |
| Author | Michael A. Innis |
| Editor | Michael A. Innis |
| Edition | illustrated |
| Publisher | Academic Press, 1990 |
| Original from | the University of Michigan |
| Digitized | 26 Jul 2008 |
| ISBN | 0123721806, 9780123721808 |
| Length | 482 pages |
| Subjects | › › › DNA Polymerases Gene Amplification - methods Gene amplification Genetic Engineering - methods Medical / Laboratory Medicine Polymerase chain reaction RNA Polymerases Science / Chemistry / General Science / Life Sciences / Biology / Molecular Biology Science / Life Sciences / Cytology Science / Life Sciences / Genetics & Genomics |
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