PCR Protocols: A Guide to Methods and ApplicationsMichael A. Innis The broad utility of the polymerase chain reaction (PCR) method is now within the reach of every researcher. Designed for use at the laboratory bench, this is the most comprehensive manual on PCR available today. Over 50 chapters provide precise instructions on procedures, with advice on primer design. All of the techniques described, from amplification and direct sequencing of genomic DNA through cDNA cloning and quantitation of mRNA, are tested, current, and supplemented with helpful notes and illustrations. You'll also learn how to: * Optimize novel applications * Avoid many cumbersome molecular biological techniques * Set up the laboratory to avoid contamination |
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Page 462
... procedure , 222 estimated costs , 452-453 genital human papillomavirus DNA sequences Ll consensus primers for , 356-359 PCR procedure , 359-361 genomic DNA buffers , 16 cycling procedures , 17 automated , 19 manual , 18-19 ...
... procedure , 222 estimated costs , 452-453 genital human papillomavirus DNA sequences Ll consensus primers for , 356-359 PCR procedure , 359-361 genomic DNA buffers , 16 cycling procedures , 17 automated , 19 manual , 18-19 ...
Page 465
... procedure , 63-64 reagents , 62 reverse transcription , 62-63 Complementary DNA cloning with degenerate primers ( MOPAC procedure ) first - strand cDNA synthesis , 51 fractionation of products , 52-53 with Klenow fragment , 51-52 ...
... procedure , 63-64 reagents , 62 reverse transcription , 62-63 Complementary DNA cloning with degenerate primers ( MOPAC procedure ) first - strand cDNA synthesis , 51 fractionation of products , 52-53 with Klenow fragment , 51-52 ...
Page 468
... procedure , 215-216 perpendicular gel approach , 208 perpendicular procedure , 216-217 preparation of PCR - amplified samples , 213-215 ras - specific sequences , 387-388 single - stranded DNA production by asymmetric PCR , 80 HLA class ...
... procedure , 215-216 perpendicular gel approach , 208 perpendicular procedure , 216-217 preparation of PCR - amplified samples , 213-215 ras - specific sequences , 387-388 single - stranded DNA production by asymmetric PCR , 80 HLA class ...
Contents
Amplification of Genomic DNA | 13 |
Amplification of RNA | 21 |
Rapid Amplification of cDNA Ends | 28 |
Copyright | |
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Common terms and phrases
1990 by Academic Acad Academic Press agarose gel aliquots alleles amplification products amplified DNA analysis annealing Applications Copyright assay cDNA cells chromosome cloning concentration containing cycles denaturation detection digestion DNA fragments DNA sequences dNTP dot blot EDTA electrophoresis enzymatic amplification ethanol ethidium bromide extraction Faloona form reserved gene genetic genomic genomic DNA Guide to Methods H. A. Erlich Higuchi hybridization Incubate labeled lanes Literature Cited membrane Methods and Applications MgCl2 minutes molecular molecules mRNA mutations Natl Nucleic Acids Res nucleotide oligomer oligonucleotide probes PCR amplification PCR primers PCR product PCR Protocols Perkin-Elmer Cetus phage pmol polymerase chain reaction polymorphisms primer primer pair procedure purified quences reagents recombinant region restriction enzymes reverse transcriptase rights of reproduction rRNA Saiki Scharf single-stranded Sninsky solution Southern blot specific strand synthesis Taq DNA polymerase Taq polymerase temperature template tion tissue transcription Tris-HCl pH tube virus vitro