PCR Protocols: A Guide to Methods and ApplicationsMichael A. Innis The broad utility of the polymerase chain reaction (PCR) method is now within the reach of every researcher. Designed for use at the laboratory bench, this is the most comprehensive manual on PCR available today. Over 50 chapters provide precise instructions on procedures, with advice on primer design. All of the techniques described, from amplification and direct sequencing of genomic DNA through cDNA cloning and quantitation of mRNA, are tested, current, and supplemented with helpful notes and illustrations. You'll also learn how to: * Optimize novel applications * Avoid many cumbersome molecular biological techniques * Set up the laboratory to avoid contamination |
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Page 77
... stranded PCR products ( Wrischnik et al . 1987 ; Higuchi et al . 1988 ; Newton et al . 1988 ) , the protocols available for preparing double - stranded template DNA for sequencing were developed ... Single - Stranded DNA by Asymmetric PCR 77.
... stranded PCR products ( Wrischnik et al . 1987 ; Higuchi et al . 1988 ; Newton et al . 1988 ) , the protocols available for preparing double - stranded template DNA for sequencing were developed ... Single - Stranded DNA by Asymmetric PCR 77.
Page 82
... single - stranded DNA ( Gyllensten and Erlich 1988 ) . A total cycle number between 30 and 40 will give the best results with most targets . Variability in the single - stranded DNA yield and background level of the PCR has been ...
... single - stranded DNA ( Gyllensten and Erlich 1988 ) . A total cycle number between 30 and 40 will give the best results with most targets . Variability in the single - stranded DNA yield and background level of the PCR has been ...
Page 480
... single - stranded products of asymmetric PCR , 194–195 rRNA genes ( mitochondrial , chloroplast ) , 312 with Taq DNA polymerase annealing template and primer , 192-193 labeling reaction , 193 reagents , 191-192 single - stranded ...
... single - stranded products of asymmetric PCR , 194–195 rRNA genes ( mitochondrial , chloroplast ) , 312 with Taq DNA polymerase annealing template and primer , 192-193 labeling reaction , 193 reagents , 191-192 single - stranded ...
Contents
Amplification of Genomic DNA | 13 |
Amplification of RNA | 21 |
Rapid Amplification of cDNA Ends | 28 |
Copyright | |
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Common terms and phrases
1990 by Academic Acad Academic Press agarose gel aliquots alleles amplification products amplified DNA analysis annealing Applications Copyright assay cDNA cells chromosome cloning concentration containing cycles denaturation detection digestion DNA fragments DNA sequences dNTP dot blot EDTA electrophoresis enzymatic amplification ethanol ethidium bromide extraction Faloona form reserved gene genetic genomic genomic DNA Guide to Methods H. A. Erlich Higuchi hybridization Incubate labeled lanes Literature Cited membrane Methods and Applications MgCl2 minutes molecular molecules mRNA mutations Natl Nucleic Acids Res nucleotide oligomer oligonucleotide probes PCR amplification PCR primers PCR product PCR Protocols Perkin-Elmer Cetus phage pmol polymerase chain reaction polymorphisms primer primer pair procedure purified quences reagents recombinant region restriction enzymes reverse transcriptase rights of reproduction rRNA Saiki Scharf single-stranded Sninsky solution Southern blot specific strand synthesis Taq DNA polymerase Taq polymerase temperature template tion tissue transcription Tris-HCl pH tube virus vitro