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a-helix acid angle axial ratio Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil complex conformation constant contribution Cotton effects creatine kinase denatured determined dialyzate diffusible components dilute electron density ellipticity enzyme equation equilibrium exciton experimental factor function gm/ml grams helical content helix hemocyanin hemoglobin I.MI intensity interaction kinase Kratky l.MI l.Mt macromolecules magnetic measurements membrane method mixture Moffitt molal molecular weight molecule myoglobin negative negative band nondiffusible number of moles observed obtained optical activity osmometers osmotic pressure paramagnetic parameter partial specific volume particles peptide Phys Pilz plot polymer polypeptides positive procedure protein component protein solution pulse radius of gyration random coil residues rotational strength rotatory sample Schellman Section shape small-angle scattering solvent solvent medium spectrum sphere structure studies subunits techniques temperature theoretical scattering curves Tinoco tion transition wavelength zero