Physical Principles and Techniques of Protein ChemistrySydney J. Leach Physical Principles and Techniques of Protein Chemistry Part C ... |
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Page 17
... density measurement can be used to fix the composition of the mixture with very little uncertainty . If density - composition tables or relations are already available at a desired temperature for a particular solute in water , a highly ...
... density measurement can be used to fix the composition of the mixture with very little uncertainty . If density - composition tables or relations are already available at a desired temperature for a particular solute in water , a highly ...
Page 35
... density of an aqueous solution is increased by about 0.003 gm / ml ( the density increment ) for each 1 % increase in the concentration of a protein . ( Note : salts , buffer ions , and many other solutes in relatively large amounts in ...
... density of an aqueous solution is increased by about 0.003 gm / ml ( the density increment ) for each 1 % increase in the concentration of a protein . ( Note : salts , buffer ions , and many other solutes in relatively large amounts in ...
Page 157
... density is negligible at small angles , it causes some variation in the scattering function at large angles ... density . By measuring pro- teins in solvents of different electron densities , the whole scattering function can be split ...
... density is negligible at small angles , it causes some variation in the scattering function at large angles ... density . By measuring pro- teins in solvents of different electron densities , the whole scattering function can be split ...
Contents
Density and Volume Change Measurements | 1 |
Osmotic Pressure | 18 |
Optical Rotatory | 22 |
Copyright | |
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a-helix absorption acid angle axial ratio B-structure band Biochem Biochemistry Biol buffer c₂ calculated CD spectra Chem chromophore coefficient Cohn complex concentration conformation constant Cotton effects creatine kinase cross-section curves denaturation determined dialyzate diffusible components dilute electron density ellipsoids ellipticity enzyme equation equilibrium exciton experimental factor function grams Guinier helical helix hemocyanin hemoglobin intensity interaction investigations kinase Kratky lysozyme macromolecule magnetic measurements membrane method mg/ml molal molecular weight molecule myoglobin negative nondiffusible observed obtained optical activity osmometers osmotic pressure paramagnetic parameter partial specific volume particles peptide Phys Pilz plot polymer polypeptides Porod primary beam procedure protein protein solution pulse radii radius of gyration residues rotational strength sample Schellman Section shape small-angle scattering solvent solvent medium spectrum sphere structure studies subunits T₁ temperature theoretical scattering curves Tinoco tion transition wavelength zero