Forensic DNA Profiling Protocols

Front Cover
Patrick J. Lincoln, Jim Thomson
Springer Science & Business Media, Jan 22, 1998 - Medical - 309 pages
It is now more than ten years since Dr. Alec Jeffreys (now Professor Sir Alec Jeffreys, FRS) reported in Nature that the investigation of certain minisatellite regions in the human genome could produce what he termed DNA fingerprints and provide useful information in the fields of paternity testing and forensic analysis. Since that time we have witnessed a revolution in the field of forensic identification. A total change of technology, from serological or electrophoretic analysis of protein polymorphisms to direct investigation of the underlying DNA polymorphisms has occurred in a short space of time. In addition, the evolution and development of the DNA systems themselves has been rapid and spectacular. In the last decade we have progressed from the multilocus DNA fing- prints, through single locus systems based on the same Southern blot RFLP technology, to a host of systems based on the PCR technique. These include Allele Specific Oligonucleotide (ASO)-primed systems detected by dot blots, the “binary” genotypes produced by mapping variations within VNTR repeats demonstrated by minisatellite variant repeat (MVR) analysis, and yet other fragment-length polymorphisms in the form of Short Tandem Repeat (STR) loci. Hand in hand with the increasing range of systems available has been the development of new instrumentation to facilitate their analysis and allow us to explore the possibilities of high volume testing in the form of mass scre- ing and offender databases.
 

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Contents

retation and Statistical Evaluation of Multilocus
97
Brinkmann 105
118
sis of Amplified FragmentLength Polymorphisms VNTRSTR
155
fication of Short Tandem Repeat Loci Using
172
al Electrophoretic Methods for Genotyping Amplified STR Loci
181
typing STR Loci Using an Automated DNA Sequencer
193
ation of PCR Fragments by Means of Direct Blotting
209
atellite Variant Repeat Unit Mapping Using PCR MVRPCR
225
letermination by PCR Analysis of the XY Amelogenin Gene
245
es Determination by Analysis of the Cytochrome b Gene
257
f the AmpliType PM + HLA DQA1 PCR Amplification
267
Ise of Capillary Electrophoresis in Genotyping STR Loci
279
Phase Minisequencing as a Tool to Detect DNA Polymorphism
297
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