Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
From inside the book
Results 1-5 of 78
Page 8
... first determined by dry weight analysis. If the solvent medium is not pure water, its composition must be adjusted on the balance, e.g., by adding nonwater solvent components, in order to compensate for the amount of water added with ...
... first determined by dry weight analysis. If the solvent medium is not pure water, its composition must be adjusted on the balance, e.g., by adding nonwater solvent components, in order to compensate for the amount of water added with ...
Page 28
... first dilatometer, the volumes VI13 and VIA are defined as VIB = [$1071.13 + $672,131 + [Eli-'13] VIA = glzvle + gafi'z,A + gn'n,' where the bracketed terms designate the volumes occupied by the separated phases before mixing; in ...
... first dilatometer, the volumes VI13 and VIA are defined as VIB = [$1071.13 + $672,131 + [Eli-'13] VIA = glzvle + gafi'z,A + gn'n,' where the bracketed terms designate the volumes occupied by the separated phases before mixing; in ...
Page 29
... first dilatometer (process I) should show no volume change (AVI = 0) within experimental error. We might also have conducted a fourth experiment, with volumes identical to those in processes I and III, where the two limbs contained the ...
... first dilatometer (process I) should show no volume change (AVI = 0) within experimental error. We might also have conducted a fourth experiment, with volumes identical to those in processes I and III, where the two limbs contained the ...
Page 39
... first applied the principle of suspending magnetically a ferromagnetic float or buoy in solutions to obtain their density. The magnetic force, measured as electrical current to a solenoid, balances any difference in the opposing efiects ...
... first applied the principle of suspending magnetically a ferromagnetic float or buoy in solutions to obtain their density. The magnetic force, measured as electrical current to a solenoid, balances any difference in the opposing efiects ...
Page 46
... first inquire: “Why is there a variety of significantly different values of v. (or ¢2, usually) reported for protein X?” Values of v. differing by more than 5% are found in the literature for some of the best characterized proteins. [We ...
... first inquire: “Why is there a variety of significantly different values of v. (or ¢2, usually) reported for protein X?” Values of v. differing by more than 5% are found in the literature for some of the best characterized proteins. [We ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
Other editions - View all
Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero