Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page 8
... fits the data is then prepared with the aid of a desk computer or a standard program at a computer center (see also Chapter 24). Ordinarily, a rectilinear fit is observed with protein solutions, in which case a simple calculator ...
... fits the data is then prepared with the aid of a desk computer or a standard program at a computer center (see also Chapter 24). Ordinarily, a rectilinear fit is observed with protein solutions, in which case a simple calculator ...
Page 18
... fit the density—composition data for two-component solvents at a temperature of interest. One procedure is to fit a curve to reciprocal density values versus the weight fraction of solute (Lewis and Randall, 1961). The generated curve ...
... fit the density—composition data for two-component solvents at a temperature of interest. One procedure is to fit a curve to reciprocal density values versus the weight fraction of solute (Lewis and Randall, 1961). The generated curve ...
Page 29
... fitting and subsequent calculation of the quantities {1'1 and {/3 from such data (Section II,F), and by substituting the appropriate values of v', into the relations for process II, the 17. DENSITY AND VOLUME MEASUREMENTS 29.
... fitting and subsequent calculation of the quantities {1'1 and {/3 from such data (Section II,F), and by substituting the appropriate values of v', into the relations for process II, the 17. DENSITY AND VOLUME MEASUREMENTS 29.
Page 36
... fit is made to obtain the limiting slope (8p/6c2)n,°. Ordinarily, a higher concentration (2 to 5%) of the protein is used, if available, for the stock solution so that good precision may be obtained with the diluted samples. With a ...
... fit is made to obtain the limiting slope (8p/6c2)n,°. Ordinarily, a higher concentration (2 to 5%) of the protein is used, if available, for the stock solution so that good precision may be obtained with the diluted samples. With a ...
Page 41
... fitting, as pointed out in Section II,F. [In this connection, it is very timely to press for a new table of absolute ... fit (r 20.99999) are routinely obtained assuming a straight-line relationship for p vs. 02 when the protein ...
... fitting, as pointed out in Section II,F. [In this connection, it is very timely to press for a new table of absolute ... fit (r 20.99999) are routinely obtained assuming a straight-line relationship for p vs. 02 when the protein ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero