Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
From inside the book
Results 1-5 of 77
Page 18
... calculated from the urea—water relation of Kawahara and Tanford (1966) based on the data of Gucker et al. (1938) ... calculate values for the partial specific volumes of the two components at each composition interval (Godschalk, 1968) ...
... calculated from the urea—water relation of Kawahara and Tanford (1966) based on the data of Gucker et al. (1938) ... calculate values for the partial specific volumes of the two components at each composition interval (Godschalk, 1968) ...
Page 19
... calculate a derived density p'D for the solvent components in the protein solution after 02 is measured. The derived density is obtained by subtracting the number of grams ... calculated, c3 = p — 02 17. DENSITY AND VOLUME MEASUREMENTS 19.
... calculate a derived density p'D for the solvent components in the protein solution after 02 is measured. The derived density is obtained by subtracting the number of grams ... calculated, c3 = p — 02 17. DENSITY AND VOLUME MEASUREMENTS 19.
Page 20
... calculating c1 and 03 with Eqs. (29), a value of s. may be calculated with the use of Eq. (8) for comparison with the value of '7. determined directly. A discrepancy here reflects error primarily in the value assigned to 02 via an ...
... calculating c1 and 03 with Eqs. (29), a value of s. may be calculated with the use of Eq. (8) for comparison with the value of '7. determined directly. A discrepancy here reflects error primarily in the value assigned to 02 via an ...
Page 22
... calculated (cf. Kauzmann, 1959). A recent application of this method, including comparison with direct volume change measurements, is described by Noguchi and Yang (1963). The present discussion is restricted to direct volume change ...
... calculated (cf. Kauzmann, 1959). A recent application of this method, including comparison with direct volume change measurements, is described by Noguchi and Yang (1963). The present discussion is restricted to direct volume change ...
Page 29
... calculated. By curve fitting and subsequent calculation of the quantities {1'1 and {/3 from such data (Section II,F), and by substituting the appropriate values of v', into the relations for process II, the 17. DENSITY AND VOLUME ...
... calculated. By curve fitting and subsequent calculation of the quantities {1'1 and {/3 from such data (Section II,F), and by substituting the appropriate values of v', into the relations for process II, the 17. DENSITY AND VOLUME ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
Other editions - View all
Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero