Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page 4
... coefficients in the total difl'erential of the extensive (i.e., mass-dependent) property being considered. Thus, for the volume property of a solution, which is a function only of the masses of the components present at a fixed ...
... coefficients in the total difl'erential of the extensive (i.e., mass-dependent) property being considered. Thus, for the volume property of a solution, which is a function only of the masses of the components present at a fixed ...
Page 5
... coefficients within parentheses are called partial molal free energies or chemical potentials and are denoted ... coefficient ,1..- and that at some chosen reference state a.“ are used in practice. Since no such limitation applies ...
... coefficients within parentheses are called partial molal free energies or chemical potentials and are denoted ... coefficient ,1..- and that at some chosen reference state a.“ are used in practice. Since no such limitation applies ...
Page 21
... coefficient, such as an extinction coefficient, for a given protein may be significantly different between laboratories which use the same assay method and exercise similar precautions (see Section IV,B,3 for a procedure involving the ...
... coefficient, such as an extinction coefficient, for a given protein may be significantly different between laboratories which use the same assay method and exercise similar precautions (see Section IV,B,3 for a procedure involving the ...
Page 47
... coefficients of some refined proteins in water differ as much as 5% between laboratories; this is quite outside a reasonable margin for experimental error. Unquestionably, methods of 17. DENSITY AND VOLUME MEASUREMENTS 47.
... coefficients of some refined proteins in water differ as much as 5% between laboratories; this is quite outside a reasonable margin for experimental error. Unquestionably, methods of 17. DENSITY AND VOLUME MEASUREMENTS 47.
Page 59
... coefficient r (i.e., goodness of fit) of better than 0.9999 to a rectilinear, leastsquares fit of the data. Values of r an order of magnitude better than this have been obtained with this procedure. Obviously, the correlation coefficients ...
... coefficient r (i.e., goodness of fit) of better than 0.9999 to a rectilinear, leastsquares fit of the data. Values of r an order of magnitude better than this have been obtained with this procedure. Obviously, the correlation coefficients ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero