Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
From inside the book
Results 1-5 of 48
Page 27
... coil conformation; Bradbury et al. (1965) have studied the conformational transition of the benzylester derivative of this polypeptide as a function of temperature. In the latter experiments, a change in AV after subtraction of the ...
... coil conformation; Bradbury et al. (1965) have studied the conformational transition of the benzylester derivative of this polypeptide as a function of temperature. In the latter experiments, a change in AV after subtraction of the ...
Page 40
... coil below the solution, or a change in the light striking a tiny photodiode (Fig. 3), signals an adjustment of the current to the solenoid, in order to prevent any up or down movement. The magnetic force required to suspend the buoy is ...
... coil below the solution, or a change in the light striking a tiny photodiode (Fig. 3), signals an adjustment of the current to the solenoid, in order to prevent any up or down movement. The magnetic force required to suspend the buoy is ...
Page 42
... coils around the cell, it has been found possible to rotate the buoy slowly and steadily while in support, and thus to obtain the viscosity simultaneously with the density (Beams and Hodgins, 1971; Kupke et al., 1972). The magnetic ...
... coils around the cell, it has been found possible to rotate the buoy slowly and steadily while in support, and thus to obtain the viscosity simultaneously with the density (Beams and Hodgins, 1971; Kupke et al., 1972). The magnetic ...
Page 91
... coils, and with charged proteins which give rise to a diffusibleion imbalance, or with combinations of these. Except for the ideal diffusible-ion imbalance (see below), positive effects may be lumped together under the term solution ...
... coils, and with charged proteins which give rise to a diffusibleion imbalance, or with combinations of these. Except for the ideal diffusible-ion imbalance (see below), positive effects may be lumped together under the term solution ...
Page 107
... coil behavior of disulfide-reduced polypeptide chains in 6M guanidinium chloride. Since polypeptides in this solvent ... coils. The power of the osmotic pressure method has been exploited in depth on the longstanding enigma surrounding ...
... coil behavior of disulfide-reduced polypeptide chains in 6M guanidinium chloride. Since polypeptides in this solvent ... coils. The power of the osmotic pressure method has been exploited in depth on the longstanding enigma surrounding ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
Other editions - View all
Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero