Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page v
... Definitions and Basic Considerations . III. Direct Volume Change IV. Density Appendix A: Derivation of the Partial Specific Volume from the Density . . . . . . . . . . . Appendix B: Splitting-Up of the Parameter: Exclusion of Diflusible ...
... Definitions and Basic Considerations . III. Direct Volume Change IV. Density Appendix A: Derivation of the Partial Specific Volume from the Density . . . . . . . . . . . Appendix B: Splitting-Up of the Parameter: Exclusion of Diflusible ...
Page 1
... Definitions and Basic Considerations . . . . . . . . 3 A. The Volume Property . . 3 B.TheDensity. . . . . . . . . . . . . 6 C. The Partial Specific Volume . . . . . . . . . 8 D. The Apparent Specific Volume . . . . . . . . 10 E. The ...
... Definitions and Basic Considerations . . . . . . . . 3 A. The Volume Property . . 3 B.TheDensity. . . . . . . . . . . . . 6 C. The Partial Specific Volume . . . . . . . . . 8 D. The Apparent Specific Volume . . . . . . . . 10 E. The ...
Page 2
... definition of the protein component at osmotic equilibrium grams of difl'usiblc component j (per gram protein) which excludes any other diffusible component j relative to the composition of the diffusible components in the dialyzate ...
... definition of the protein component at osmotic equilibrium grams of difl'usiblc component j (per gram protein) which excludes any other diffusible component j relative to the composition of the diffusible components in the dialyzate ...
Page 3
... define protein solutions routinely in terms of the partial volumes of all the components present in a system. With a knowledge of the density p of a protein solution, the volume V may be obtained, since by definition the density is mass ...
... define protein solutions routinely in terms of the partial volumes of all the components present in a system. With a knowledge of the density p of a protein solution, the volume V may be obtained, since by definition the density is mass ...
Page 4
... defined as the change in total volume per unit mass upon adding an infinitesimal amount of component i at constant temperature T, pressure P, and masses in grams g,- of all other components 7'. Thus _ _ g . . v1 — (agilhi (.7 ¢ 7I) (2) ...
... defined as the change in total volume per unit mass upon adding an infinitesimal amount of component i at constant temperature T, pressure P, and masses in grams g,- of all other components 7'. Thus _ _ g . . v1 — (agilhi (.7 ¢ 7I) (2) ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero