Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page 25
... denaturation reactions gave answers for the overall AV, characteristic of the processes. The number of distinct chemical events was often rather large and precluded any precise correlations being drawn between volume changes and the ...
... denaturation reactions gave answers for the overall AV, characteristic of the processes. The number of distinct chemical events was often rather large and precluded any precise correlations being drawn between volume changes and the ...
Page 27
... denaturation of serum albumin, using a highly precise dilatometric protocol, has been studied as a function of urea and guanidinium chloride concentration (Katz and Ferris, 1966; Katz, 1968). In these experiments, A¢2 was determined ...
... denaturation of serum albumin, using a highly precise dilatometric protocol, has been studied as a function of urea and guanidinium chloride concentration (Katz and Ferris, 1966; Katz, 1968). In these experiments, A¢2 was determined ...
Page 28
... denaturing agent, which is called component 3. We will consider three processes (I, II, and III) in three separate dilatometers. In one dilatometer, a water solution of the protein at a sufficiently high concentration is placed in one ...
... denaturing agent, which is called component 3. We will consider three processes (I, II, and III) in three separate dilatometers. In one dilatometer, a water solution of the protein at a sufficiently high concentration is placed in one ...
Page 49
... denaturation process (Katz and Ferris, 1966); the change in the partial specific volume Of the denaturing agent (Airs) at a fixed composition of protein and water may be highly instructive also. A wide spectrum of opportunity in this ...
... denaturation process (Katz and Ferris, 1966); the change in the partial specific volume Of the denaturing agent (Airs) at a fixed composition of protein and water may be highly instructive also. A wide spectrum of opportunity in this ...
Page 61
... denaturation as a function Of pressure is illustrated by the findings and discussions concerning the role of hydrophobic bonds in proteins (Kliman, 1969; Brandts et al., 1970). The usefulness of density measurements for studying the ...
... denaturation as a function Of pressure is illustrated by the findings and discussions concerning the role of hydrophobic bonds in proteins (Kliman, 1969; Brandts et al., 1970). The usefulness of density measurements for studying the ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero