Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page 7
... determine the concentration of a pair of nonvolatile components, such as protein and salt, in an aqueous mixture by a ... determined on this solution is p = (2g,)/V. Hence, a value for the total volume is given by the ratio (2g,)/p. From ...
... determine the concentration of a pair of nonvolatile components, such as protein and salt, in an aqueous mixture by a ... determined on this solution is p = (2g,)/V. Hence, a value for the total volume is given by the ratio (2g,)/p. From ...
Page 8
... determination of the dry weight of the twocomponent solvent medium, usually carried out concurrently with that of the ... determined by dry weight analysis. If the solvent medium is not pure water, its composition must be adjusted on the ...
... determination of the dry weight of the twocomponent solvent medium, usually carried out concurrently with that of the ... determined by dry weight analysis. If the solvent medium is not pure water, its composition must be adjusted on the ...
Page 11
... of the protein becomes identical. The value of v,- at any concentration of component i may be determined, as noted before, by taking the limiting slope to a fixed value of 0.. In this case, 17. DENSITY AND VOLUME MEASUREMENTS 11.
... of the protein becomes identical. The value of v,- at any concentration of component i may be determined, as noted before, by taking the limiting slope to a fixed value of 0.. In this case, 17. DENSITY AND VOLUME MEASUREMENTS 11.
Page 12
... determined at each concentration; in fact, such 452 may vary over a concentration range in which {,2 is constant ... determine the apparent specific volume of the solute of interest, usually at low concentrations. It is not useful, then ...
... determined at each concentration; in fact, such 452 may vary over a concentration range in which {,2 is constant ... determine the apparent specific volume of the solute of interest, usually at low concentrations. It is not useful, then ...
Page 13
... determine 952 at each 02, information is required on the changing mass ratios of the difiusible components in each ... determined instead of v' (or v°' at 02 = O). v°' for a nondiffusible component is defined by a relation similar to ...
... determine 952 at each 02, information is required on the changing mass ratios of the difiusible components in each ... determined instead of v' (or v°' at 02 = O). v°' for a nondiffusible component is defined by a relation similar to ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero