Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page vi
... Experimental Methods V. Experimental Design VI. Individual Enzymes . . . . . . . . VII. Absorption of Water by Diamagnetic Molecules . References . . . 21. The Use of Least Squares in Data Analysis R. D. B. Fraser and E. Suzuki Glossary ...
... Experimental Methods V. Experimental Design VI. Individual Enzymes . . . . . . . . VII. Absorption of Water by Diamagnetic Molecules . References . . . 21. The Use of Least Squares in Data Analysis R. D. B. Fraser and E. Suzuki Glossary ...
Page 6
... experimental values, reduced to the same dimensions, is usually quite small, the difference of about 3 parts in 10' obtained by intermingling the volume units becomes quite important when calibrating a procedure with known standards ...
... experimental values, reduced to the same dimensions, is usually quite small, the difference of about 3 parts in 10' obtained by intermingling the volume units becomes quite important when calibrating a procedure with known standards ...
Page 26
... experimental results and associative interpretations. By dilatometry also, the volume change for the formation of water from hydrogen and hydroxyl ions was reevaluated (Bodanszky and Kauzmann, 1962) in order to interpret more accurately ...
... experimental results and associative interpretations. By dilatometry also, the volume change for the formation of water from hydrogen and hydroxyl ions was reevaluated (Bodanszky and Kauzmann, 1962) in order to interpret more accurately ...
Page 27
... experiments, a change in AV after subtraction of the expansion Of the solvent (dichloroacetic acid—dichloroethane, 3 ... experimental uncertainties, the results suggest that the partial volumes of the solvent components changed also. In ...
... experiments, a change in AV after subtraction of the expansion Of the solvent (dichloroacetic acid—dichloroethane, 3 ... experimental uncertainties, the results suggest that the partial volumes of the solvent components changed also. In ...
Page 29
... experiment with the first dilatometer (process I) should show no volume change (AVI = 0) within experimental error. We might also have conducted a fourth experiment, with volumes identical to those in processes I and III, where the two ...
... experiment with the first dilatometer (process I) should show no volume change (AVI = 0) within experimental error. We might also have conducted a fourth experiment, with volumes identical to those in processes I and III, where the two ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero