Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page 1
... grams per milliliter of a component f,~ weight fraction of a species j in component 1' relative to the total component g grams of a component g acceleration of gravity 1' any component in the solution j any nonprotein component (usually ...
... grams per milliliter of a component f,~ weight fraction of a species j in component 1' relative to the total component g grams of a component g acceleration of gravity 1' any component in the solution j any nonprotein component (usually ...
Page 2
... grams of component j (per gram protein) transported across a semipermeable membrane during dialysis chemical potential microliter grams of component j (per gram protein) included in the definition of the protein component at osmotic ...
... grams of component j (per gram protein) transported across a semipermeable membrane during dialysis chemical potential microliter grams of component j (per gram protein) included in the definition of the protein component at osmotic ...
Page 3
... grams g of each component i in a milieu of N components at a specified temperature and pressure is V = i Tag-- (1) where V is in milliliters and v. is the partial i=1 17. DENSITY AND VOLUME MEASUREMENTS 3 II. Definitions and Basic ...
... grams g of each component i in a milieu of N components at a specified temperature and pressure is V = i Tag-- (1) where V is in milliliters and v. is the partial i=1 17. DENSITY AND VOLUME MEASUREMENTS 3 II. Definitions and Basic ...
Page 4
... grams g,- of all other components 7'. Thus _ _ g . . v1 — (agilhi (.7 ¢ 7I) (2) Alternatively, one might imagine the finite change in volume after adding a measurable quantity of component i to a very large sea of the solution so that ...
... grams g,- of all other components 7'. Thus _ _ g . . v1 — (agilhi (.7 ¢ 7I) (2) Alternatively, one might imagine the finite change in volume after adding a measurable quantity of component i to a very large sea of the solution so that ...
Page 6
... grams of the component designated by a subscript and milliliters are chosen as the units of volume.“l The density Of a solution, therefore, is the sum of the masses in grams of all the components in the mixture divided by the number of ...
... grams of the component designated by a subscript and milliliters are chosen as the units of volume.“l The density Of a solution, therefore, is the sum of the masses in grams of all the components in the mixture divided by the number of ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero