Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page 30
... hemoglobin. The volume changes with time were followed until most of the protein had crystallized (15 to 20 hr). In other experiments the salt was added directly to a solution of the protein, then deaerated, and placed in the ...
... hemoglobin. The volume changes with time were followed until most of the protein had crystallized (15 to 20 hr). In other experiments the salt was added directly to a solution of the protein, then deaerated, and placed in the ...
Page 100
... has since become of general application and is utilized in a number of other kinds of equilibrium measurements in multicomponent systems. 9There appears to be substantial agreement that hemoglobin dimers are 100 M. J. KELLY AND D. w. KUPKE.
... has since become of general application and is utilized in a number of other kinds of equilibrium measurements in multicomponent systems. 9There appears to be substantial agreement that hemoglobin dimers are 100 M. J. KELLY AND D. w. KUPKE.
Page 107
... hemoglobin (Guidotti, 1967a). This study provided evidence that the unliganded tetramer (deoxyhemoglobin) is more stable to dissociation into the assumed dimeric form than the liganded tetramers (i.e., oxy-, carbomonoxy-, and ...
... hemoglobin (Guidotti, 1967a). This study provided evidence that the unliganded tetramer (deoxyhemoglobin) is more stable to dissociation into the assumed dimeric form than the liganded tetramers (i.e., oxy-, carbomonoxy-, and ...
Page 112
... hemoglobin and the reaction mechanism of hemoglobin with ligands, such as oxygen (Guidotti, 1967a,b,c). The experiments were designed to test the fundamental hypothesis that dissimilar or “unlike” dimer subunits, arising from the ...
... hemoglobin and the reaction mechanism of hemoglobin with ligands, such as oxygen (Guidotti, 1967a,b,c). The experiments were designed to test the fundamental hypothesis that dissimilar or “unlike” dimer subunits, arising from the ...
Page 113
... hemoglobin in hemoglobin mixtures, while indicated by some techniques, has been in question. The discriminating power of the osmotic pressure method was challenged in the light of the following considerations. If tetrameric hybrids are ...
... hemoglobin in hemoglobin mixtures, while indicated by some techniques, has been in question. The discriminating power of the osmotic pressure method was challenged in the light of the following considerations. If tetrameric hybrids are ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero