Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page 1
... solution j any nonprotein component (usually membrane-diffusible) except where (j a6 i) is specified <_<-<§::55 'rgq<awwwzzag~mmom~s l N 91% E < OI H. L”: 1 Chapter 17. Density and Volume Change Measurements Glossary of Symbols.
... solution j any nonprotein component (usually membrane-diffusible) except where (j a6 i) is specified <_<-<§::55 'rgq<awwwzzag~mmom~s l N 91% E < OI H. L”: 1 Chapter 17. Density and Volume Change Measurements Glossary of Symbols.
Page 3
... measurement, is quite new. The reason for this limited view on density applications is self-evident; density measurements of sufficient accuracy by the classical methods were time consuming, awkward to perform, severely limited with ...
... measurement, is quite new. The reason for this limited view on density applications is self-evident; density measurements of sufficient accuracy by the classical methods were time consuming, awkward to perform, severely limited with ...
Page 5
... one or more (perhaps all) of the added components relative to some initial state must have taken place in accordance with Eq. (1) if the total masses are constant or properly accounted for. Hence, if 17. DENSITY AND VOLUME MEASUREMENTS 5.
... one or more (perhaps all) of the added components relative to some initial state must have taken place in accordance with Eq. (1) if the total masses are constant or properly accounted for. Hence, if 17. DENSITY AND VOLUME MEASUREMENTS 5.
Page 6
... measurements are feasible, the overall AV for a process can be separated into quantities related to each component. Usually, only the partial specific volume, or its change Av, of the protein component alone is measured. Clearly ...
... measurements are feasible, the overall AV for a process can be separated into quantities related to each component. Usually, only the partial specific volume, or its change Av, of the protein component alone is measured. Clearly ...
Page 7
... measurements. A mass of the three-component solution to be dried is weighed on the balance to give Egg. The density determined on this solution is p = (2g,)/V. Hence, a value for the total volume is given by the ratio (2g,)/p. From the ...
... measurements. A mass of the three-component solution to be dried is weighed on the balance to give Egg. The density determined on this solution is p = (2g,)/V. Hence, a value for the total volume is given by the ratio (2g,)/p. From the ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero