Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
From inside the book
Results 1-5 of 83
Page 19
... method becomes useless. It is evident that a unique value for the composition is not given by a density measurement if the solution contains more than two components. Frequently, we are interested in a three-component solution, such as ...
... method becomes useless. It is evident that a unique value for the composition is not given by a density measurement if the solution contains more than two components. Frequently, we are interested in a three-component solution, such as ...
Page 20
... method may not be an expedient procedure for determining c1 and 03. Nonetheless, evaluation of v. and if. is straightforward. v1 may be obtained by weighing-in increments of water to aliquots of the equilibrated protein solution and ...
... method may not be an expedient procedure for determining c1 and 03. Nonetheless, evaluation of v. and if. is straightforward. v1 may be obtained by weighing-in increments of water to aliquots of the equilibrated protein solution and ...
Page 21
... method chosen must be insensitive to the concentration of component 3. These indirect methods, ultimately, are based on a unit of the dry mass of the protein component. Thus, the error in two analytical methods must be contended with ...
... method chosen must be insensitive to the concentration of component 3. These indirect methods, ultimately, are based on a unit of the dry mass of the protein component. Thus, the error in two analytical methods must be contended with ...
Page 22
... method, including comparison with direct volume change measurements, is described by Noguchi and Yang (1963). The present discussion is restricted to direct volume change measurements by dilatometry. A. DILATOMETRY The direct ...
... method, including comparison with direct volume change measurements, is described by Noguchi and Yang (1963). The present discussion is restricted to direct volume change measurements by dilatometry. A. DILATOMETRY The direct ...
Page 33
... methods, the volume of a small object immersed in sample liquids is required, but such volumes are either cancelled out ... method remains the simplest and least expensive procedure in terms of readily available equipment but may be the ...
... methods, the volume of a small object immersed in sample liquids is required, but such volumes are either cancelled out ... method remains the simplest and least expensive procedure in terms of readily available equipment but may be the ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
Other editions - View all
Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero