Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page 2
... moles of component j (per mole protein) included in the definition of the protein component at osmotic equilibrium I. Introduction Until recently, the potential usefulness Of density measurements for the study of proteins in solution ...
... moles of component j (per mole protein) included in the definition of the protein component at osmotic equilibrium I. Introduction Until recently, the potential usefulness Of density measurements for the study of proteins in solution ...
Page 4
... moles n for grams of mass g. If temperature and/or pressure are not held constant, the partial volumes may vary even at constant composition, because the volume is also a function of these “intensive” (i.e., mass-independent) variables ...
... moles n for grams of mass g. If temperature and/or pressure are not held constant, the partial volumes may vary even at constant composition, because the volume is also a function of these “intensive” (i.e., mass-independent) variables ...
Page 5
... moles of the components, at constant temperature and pressure, is N 6G 21 (am->11»... , n ('7 75 IL) (4) 1: where the coefficients within parentheses are called partial molal free energies or chemical potentials and are denoted usually ...
... moles of the components, at constant temperature and pressure, is N 6G 21 (am->11»... , n ('7 75 IL) (4) 1: where the coefficients within parentheses are called partial molal free energies or chemical potentials and are denoted usually ...
Page 11
... moles of the solute per kilogram of water, the apparent molal volume is obtained.) The definition implies that the solvent medium behaves ideally with respect to its volume contribution. The solvent, of course, cannot be assigned a ...
... moles of the solute per kilogram of water, the apparent molal volume is obtained.) The definition implies that the solvent medium behaves ideally with respect to its volume contribution. The solvent, of course, cannot be assigned a ...
Page 15
... moles of excess, or of deficient, diffusible component per mole of the anhydrous protein remains constant over the range in c2“ under investigation. Ordinarily, the change in osmotic pressure with 02* is <<1 atm, yielding a trivial ...
... moles of excess, or of deficient, diffusible component per mole of the anhydrous protein remains constant over the range in c2“ under investigation. Ordinarily, the change in osmotic pressure with 02* is <<1 atm, yielding a trivial ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero