Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page 2
... negative, respectively) axial distance from solenoid core internal energy Gibbs free energy enthalpy magnetic field intensity electric current (amperes) molecular .weight magnetic moment total number of components in a macrophase number ...
... negative, respectively) axial distance from solenoid core internal energy Gibbs free energy enthalpy magnetic field intensity electric current (amperes) molecular .weight magnetic moment total number of components in a macrophase number ...
Page 15
... negative) so that 02' = 02 + EM (1' r5 2) (20) where 5,- is the grams of excess (or deficient) diffusible component j per gram of the anhydrous protein. For example, if component 3 is the excess diffusible component, the amount .53 must ...
... negative) so that 02' = 02 + EM (1' r5 2) (20) where 5,- is the grams of excess (or deficient) diffusible component j per gram of the anhydrous protein. For example, if component 3 is the excess diffusible component, the amount .53 must ...
Page 31
... negative conformational volume increment to the partial volume of the monomer when it changed to the inactive form. 4. Kinetic Experiments The rate of change of volume during a process can often be followed quite easily by carefully ...
... negative conformational volume increment to the partial volume of the monomer when it changed to the inactive form. 4. Kinetic Experiments The rate of change of volume during a process can often be followed quite easily by carefully ...
Page 54
... negative. With this definition of 8 it becomes clear that the experimental protocol does not depend on maintaining either a constant volume of the protein solution or a fixed concentration of the protein during the equilibration. The ...
... negative. With this definition of 8 it becomes clear that the experimental protocol does not depend on maintaining either a constant volume of the protein solution or a fixed concentration of the protein during the equilibration. The ...
Page 71
... negative, while A58 is positive. This may be seen more easily by recasting Eq. (64) in terms of the concentrations in grams per milliliter where l . 0' $1 = $1 — Ea (65) c a As water becomes more concentrated, even a small value for .53 ...
... negative, while A58 is positive. This may be seen more easily by recasting Eq. (64) in terms of the concentrations in grams per milliliter where l . 0' $1 = $1 — Ea (65) c a As water becomes more concentrated, even a small value for .53 ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero