Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page 9
... obtained from the weight fraction W of the component (W.- = gi/Egj) and the density of the solution, since by definition 0. = pW.-. Hence, the alternative expression in terms of W is 6111p o _ __~0, . . (3W; )s, — (1 VH0) (J yé IL) (11b) ...
... obtained from the weight fraction W of the component (W.- = gi/Egj) and the density of the solution, since by definition 0. = pW.-. Hence, the alternative expression in terms of W is 6111p o _ __~0, . . (3W; )s, — (1 VH0) (J yé IL) (11b) ...
Page 12
... obtained which is essentially constant upon further addition of titrant. Similarly, if a volume change accompanies ... obtain the apparent specific volume of the solvent medium is often impractical. Moreover, the objective is to ...
... obtained which is essentially constant upon further addition of titrant. Similarly, if a volume change accompanies ... obtain the apparent specific volume of the solvent medium is often impractical. Moreover, the objective is to ...
Page 13
... obtained rather than (Hp/802),“; the subscript ,u. denotes that each of the diffusible components has the same chemical potential in every phase. The values of (tip/502)” may be quite different from the values (Biz/Bog)". at the same ...
... obtained rather than (Hp/802),“; the subscript ,u. denotes that each of the diffusible components has the same chemical potential in every phase. The values of (tip/502)” may be quite different from the values (Biz/Bog)". at the same ...
Page 17
... obtained by simply referring the observed density to the table. Density—composition tables may be available to an ... obtaining accurate weights (e.g., Corwin, 1959; Macurdy, 1967). For many studies on protein solutions an accuracy of 10 ...
... obtained by simply referring the observed density to the table. Density—composition tables may be available to an ... obtaining accurate weights (e.g., Corwin, 1959; Macurdy, 1967). For many studies on protein solutions an accuracy of 10 ...
Page 19
... obtained if an independent evaluation Of the protein concentration is carried out and the partial specific volume of the protein is known. Where (Sp/beg)“, is found to be constant during the determination of V2, as is usually the case ...
... obtained if an independent evaluation Of the protein concentration is carried out and the partial specific volume of the protein is known. Where (Sp/beg)“, is found to be constant during the determination of V2, as is usually the case ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero