Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page 27
... polymers: overlap of electrostatic fields between ionic groups which change with pH, hydrogen bonding between polymer residues and between residues and water during helix to random-coil transitions, the change in pK of the dissociable ...
... polymers: overlap of electrostatic fields between ionic groups which change with pH, hydrogen bonding between polymer residues and between residues and water during helix to random-coil transitions, the change in pK of the dissociable ...
Page 30
... polymerization process appears to be a monomer addition to pre-existing short filaments or seed polymer, reminiscent of glycogen formation. Neither the seeds nor the monomer polymerize in the absence of the other under the conditions ...
... polymerization process appears to be a monomer addition to pre-existing short filaments or seed polymer, reminiscent of glycogen formation. Neither the seeds nor the monomer polymerize in the absence of the other under the conditions ...
Page 31
... polymer in the identical solvent medium. On mixing the phases, polymerization proceeded rapidly to completion (about 30 min), depending on the temperature. Since a change in conformation of the flagellin was held to be unlikely, or at ...
... polymer in the identical solvent medium. On mixing the phases, polymerization proceeded rapidly to completion (about 30 min), depending on the temperature. Since a change in conformation of the flagellin was held to be unlikely, or at ...
Page 32
... polymer growth is initiated simply by the introduction of some seed polymer as nucleating agent without altering the solvent medium. [The effect of diluting the fiagellin upon addition of the seeding solution was negligible; i.e., (a¢g ...
... polymer growth is initiated simply by the introduction of some seed polymer as nucleating agent without altering the solvent medium. [The effect of diluting the fiagellin upon addition of the seeding solution was negligible; i.e., (a¢g ...
Page 44
... polymers were reported. By the use of stronger materials, it was found possible to measure density difierences under pressures up to 10 atm and at temperatures up to 230°C; the accuracy, however, was limited to about 10" gm/cm3 because ...
... polymers were reported. By the use of stronger materials, it was found possible to measure density difierences under pressures up to 10 atm and at temperatures up to 230°C; the accuracy, however, was limited to about 10" gm/cm3 because ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero