Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Results 1-5 of 68
Page 15
... (positive or negative) so that 02' = 02 + EM (1' r5 2) (20) where 5,- is the grams of excess (or deficient) diffusible component j per gram of the anhydrous protein. For example, if component 3 is the excess diffusible component, the ...
... (positive or negative) so that 02' = 02 + EM (1' r5 2) (20) where 5,- is the grams of excess (or deficient) diffusible component j per gram of the anhydrous protein. For example, if component 3 is the excess diffusible component, the ...
Page 27
... positive value found during this selfassociation is in substantial agreement with other evidence supporting a mechanism for the release of bound water from the subunits. The change in ¢2 with denaturation of serum albumin, using a ...
... positive value found during this selfassociation is in substantial agreement with other evidence supporting a mechanism for the release of bound water from the subunits. The change in ¢2 with denaturation of serum albumin, using a ...
Page 55
... positive values of the parameter 5, as well as of 8, do not imply that a preferred component is bound or interacts with protein while the ejected component does not interact. In more general terms, both diffusible components could ...
... positive values of the parameter 5, as well as of 8, do not imply that a preferred component is bound or interacts with protein while the ejected component does not interact. In more general terms, both diffusible components could ...
Page 71
... (positive) values for both £3 and 53*, which are held constant over a difference in W'a, values of A5, or A58 are not zero. For example, in proceeding from a dialyzate with a higher concentration of component 3 to one where W's is lower ...
... (positive) values for both £3 and 53*, which are held constant over a difference in W'a, values of A5, or A58 are not zero. For example, in proceeding from a dialyzate with a higher concentration of component 3 to one where W's is lower ...
Page 91
... Positive departure from the 1r vs. w, proportionality is usually manifested by a constant value of B in Eqs. (18), as indicated in curve (a). This kind of plot is seen with mixtures of nonaggregating proteins, with proteins which are ...
... Positive departure from the 1r vs. w, proportionality is usually manifested by a constant value of B in Eqs. (18), as indicated in curve (a). This kind of plot is seen with mixtures of nonaggregating proteins, with proteins which are ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero