Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page xi
... protein to protein is, to say the least, imperfect. Yet these interactions and their molecular efiects are central to our understanding of the many facets of protein function. Certainly there has been a shift from the picture of a rigid ...
... protein to protein is, to say the least, imperfect. Yet these interactions and their molecular efiects are central to our understanding of the many facets of protein function. Certainly there has been a shift from the picture of a rigid ...
Page xiii
... Proteins 1. Dielectric Relaxation Shiro Takashima Dielectric Properties of Proteins 11. Electric Birefringence and Dichroism Yoshiro Yoshioka and Hiroshi Watanabe Electrophoresis John R. Cann Analytical Gel Filtration D. J. Winzor ...
... Proteins 1. Dielectric Relaxation Shiro Takashima Dielectric Properties of Proteins 11. Electric Birefringence and Dichroism Yoshiro Yoshioka and Hiroshi Watanabe Electrophoresis John R. Cann Analytical Gel Filtration D. J. Winzor ...
Page 2
... protein) transported across a semipermeable membrane during dialysis chemical potential microliter grams of component j (per gram protein) included in the definition of the protein component at osmotic equilibrium grams of difl'usiblc ...
... protein) transported across a semipermeable membrane during dialysis chemical potential microliter grams of component j (per gram protein) included in the definition of the protein component at osmotic equilibrium grams of difl'usiblc ...
Page 3
... proteins has provided the principal impetus for determining the density of protein solutions accurately. The application of density to other purposes, as a. routine measurement, is quite new. The reason for this limited view on density ...
... proteins has provided the principal impetus for determining the density of protein solutions accurately. The application of density to other purposes, as a. routine measurement, is quite new. The reason for this limited view on density ...
Page 6
... proteins and other macromolecules. The acquisition of detailed background information on the changes in total volume of simpler systems has been initiated as an approach to a sound interpretation of the volume property in protein ...
... proteins and other macromolecules. The acquisition of detailed background information on the changes in total volume of simpler systems has been initiated as an approach to a sound interpretation of the volume property in protein ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero