Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page 7
... residue comprising the combined masses of protein and salt. Recalling Eqs. (1) and (9), it is evident that two simultaneous equations exist for calculating g2 and g, in the dried residue if the partial specific volumes are available for ...
... residue comprising the combined masses of protein and salt. Recalling Eqs. (1) and (9), it is evident that two simultaneous equations exist for calculating g2 and g, in the dried residue if the partial specific volumes are available for ...
Page 26
... residues with charge potential far outweighs the proportion found in proteins generally. The volume change on titrating with alkali in this case (22.3 ml/mole H“) was in rather striking agreement with that for model amines (e.g., lysine ...
... residues with charge potential far outweighs the proportion found in proteins generally. The volume change on titrating with alkali in this case (22.3 ml/mole H“) was in rather striking agreement with that for model amines (e.g., lysine ...
Page 27
... residues and between residues and water during helix to random-coil transitions, the change in pK of the dissociable groups before and after the conformational change, and electrostatic effects from increasing the concentration of ...
... residues and between residues and water during helix to random-coil transitions, the change in pK of the dissociable groups before and after the conformational change, and electrostatic effects from increasing the concentration of ...
Page 31
... residues was suggested on the basis of activation parameters and the high content of nonpolar residues. Above 28°C a much larger maximum AV was obtained, which suggested that the fiagellin monomer at these temperatures was in ...
... residues was suggested on the basis of activation parameters and the high content of nonpolar residues. Above 28°C a much larger maximum AV was obtained, which suggested that the fiagellin monomer at these temperatures was in ...
Page 196
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Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero