Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page 1
... solvent medium, usually containing difiusible components only; note that v' and ¢' are exceptions Double primes refer to the undialyzed (isomolal) protein solution c grams per milliliter of a component f,~ weight fraction of a species j ...
... solvent medium, usually containing difiusible components only; note that v' and ¢' are exceptions Double primes refer to the undialyzed (isomolal) protein solution c grams per milliliter of a component f,~ weight fraction of a species j ...
Page 3
... solvent medium may result in significantly different values of the partial specific volume. With newer procedures ... solution, the volume V may be obtained, since by definition the density is mass per unit volume. The difference in ...
... solvent medium may result in significantly different values of the partial specific volume. With newer procedures ... solution, the volume V may be obtained, since by definition the density is mass per unit volume. The difference in ...
Page 4
... mixture in terms of independently added components can always be known. The quantity v is defined as the change in ... solvent medium for the protein, such as urea, salts, and sugars.) Integration then yields Eq. (1) provided that the ...
... mixture in terms of independently added components can always be known. The quantity v is defined as the change in ... solvent medium for the protein, such as urea, salts, and sugars.) Integration then yields Eq. (1) provided that the ...
Page 8
... solvent medium itself. [It should be recognized here that the determination of the dry weight of the twocomponent solvent medium, usually carried out concurrently with that of the protein solution, can be related to the density of the ...
... solvent medium itself. [It should be recognized here that the determination of the dry weight of the twocomponent solvent medium, usually carried out concurrently with that of the protein solution, can be related to the density of the ...
Page 9
... solution; in effect, the solvent medium of density p' already contains the component for which a. is desired at concentration 0,. An alternative expression for the partial specific volume at any value of 0., but not employing p', is ...
... solution; in effect, the solvent medium of density p' already contains the component for which a. is desired at concentration 0,. An alternative expression for the partial specific volume at any value of 0., but not employing p', is ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero