Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page 17
... studies on protein solutions an accuracy of 10'll gm/ml in the density value is required; therefore, the work at the balance should yield absolute accuracies which exceed this level. Proper maintenance and an intimate knowledge of the ...
... studies on protein solutions an accuracy of 10'll gm/ml in the density value is required; therefore, the work at the balance should yield absolute accuracies which exceed this level. Proper maintenance and an intimate knowledge of the ...
Page 21
... studies where parameters are sought which involve differences between related systems, such as for the evaluation of 5 (Section IV,B,3) , the effect of error in 02 can be reduced substantially by performing the measurements on the ...
... studies where parameters are sought which involve differences between related systems, such as for the evaluation of 5 (Section IV,B,3) , the effect of error in 02 can be reduced substantially by performing the measurements on the ...
Page 25
... studies on volume change in protein chemistry. The earlier studies on following the changes in volume accompanying the hydrolysis of peptides, enzymic splitting of proteins, and denaturation reactions gave answers for the overall AV ...
... studies on volume change in protein chemistry. The earlier studies on following the changes in volume accompanying the hydrolysis of peptides, enzymic splitting of proteins, and denaturation reactions gave answers for the overall AV ...
Page 26
... studies; previously accepted values, based on other procedures at relatively high concentrations of alkali, were found to be in error by about 5%. A particularly interesting ob— servation with proteins is pertinent for illustration ...
... studies; previously accepted values, based on other procedures at relatively high concentrations of alkali, were found to be in error by about 5%. A particularly interesting ob— servation with proteins is pertinent for illustration ...
Page 30
... Studies A special feature of the dilatometer method is its amenability to experiments on proteins in the solid state. A recent protocol for measuring the volume change accompanying the dissolution of crystalline protein upon adding an ...
... Studies A special feature of the dilatometer method is its amenability to experiments on proteins in the solid state. A recent protocol for measuring the volume change accompanying the dissolution of crystalline protein upon adding an ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero