Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page 37
... Theoretical questions have been raised which may be diflicult to answer in a given case. Is the droplet truly immiscible (except for water exchange between the phases)? Are electrostatic charge effects at the interfaces negligible? Does ...
... Theoretical questions have been raised which may be diflicult to answer in a given case. Is the droplet truly immiscible (except for water exchange between the phases)? Are electrostatic charge effects at the interfaces negligible? Does ...
Page 79
... theoretical expertise. The principal limitation of the method is that low molalities of protein (<10“) cannot ... theoretical guide for the treatment of' systems containing proteins and ions. For those desiring a thorough and strict ...
... theoretical expertise. The principal limitation of the method is that low molalities of protein (<10“) cannot ... theoretical guide for the treatment of' systems containing proteins and ions. For those desiring a thorough and strict ...
Page 100
... theoretical considerations alone it is highly improbable that such additivity will be observed unless the two proteins are very much alike. Also, experimental results have shown that significantly different values of B are obtained with ...
... theoretical considerations alone it is highly improbable that such additivity will be observed unless the two proteins are very much alike. Also, experimental results have shown that significantly different values of B are obtained with ...
Page 104
... theoretical development on nonideal, self-associating protein systems. Evaluation of ¢2, however, requires the same care and effort as does the determination of the derivative 103(8p/8C2),.°; i.e., 4,2 is obtained from the average of ...
... theoretical development on nonideal, self-associating protein systems. Evaluation of ¢2, however, requires the same care and effort as does the determination of the derivative 103(8p/8C2),.°; i.e., 4,2 is obtained from the average of ...
Page 107
... theoretical aspects of this work is removed from the purpose at hand, and it suffices to say that the results for the unperturbed dimensions are in reasonable agreement with the predictions by Flory and associates (Brant and Flory, 1965 ...
... theoretical aspects of this work is removed from the purpose at hand, and it suffices to say that the results for the unperturbed dimensions are in reasonable agreement with the predictions by Flory and associates (Brant and Flory, 1965 ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero