Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page 27
... transition determined by optical rotation. The change in volume, however, disagreed in both sign and magnitude with that deduced pycnometrically for the change in apparent specific volume attending the transition. Barring experimental ...
... transition determined by optical rotation. The change in volume, however, disagreed in both sign and magnitude with that deduced pycnometrically for the change in apparent specific volume attending the transition. Barring experimental ...
Page 32
... transition between the active and inactive fiagellin monomer. This study is perhaps an ideal example of the use of dilatometry for kinetic purposes since the reaction for polymer growth is initiated simply by the introduction of some ...
... transition between the active and inactive fiagellin monomer. This study is perhaps an ideal example of the use of dilatometry for kinetic purposes since the reaction for polymer growth is initiated simply by the introduction of some ...
Page 47
... transition over the temperature range covering the reported experiments. The data of Hunter (1966) and others suggests that a difference Of 10°C affects the value of V2 by less than 1%. The effect of different solutes in the aqueous ...
... transition over the temperature range covering the reported experiments. The data of Hunter (1966) and others suggests that a difference Of 10°C affects the value of V2 by less than 1%. The effect of different solutes in the aqueous ...
Page 60
... transition in the conformation or at which self-association of the protein is encountered; the change in W, which assumes that the volume contribution of the solvent is fixed at each temperature, may be less informative than (dvz/dT) if ...
... transition in the conformation or at which self-association of the protein is encountered; the change in W, which assumes that the volume contribution of the solvent is fixed at each temperature, may be less informative than (dvz/dT) if ...
Page 248
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Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero