Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page 9
... zero refers to vanishing concentration of the 11'“ component and g,- denotes the fact that the masses of all other components are held constant. [The differentiation leads to an expression with additional terms (see Appendix A). However ...
... zero refers to vanishing concentration of the 11'“ component and g,- denotes the fact that the masses of all other components are held constant. [The differentiation leads to an expression with additional terms (see Appendix A). However ...
Page 20
... zero water addition) is evaluated from a plot of the observed densities versus the masses of added water. For $3, a second curve is prepared by weighingin component 3 to other aliquots of the protein solution and again the limiting ...
... zero water addition) is evaluated from a plot of the observed densities versus the masses of added water. For $3, a second curve is prepared by weighingin component 3 to other aliquots of the protein solution and again the limiting ...
Page 25
... zero time more accurately by correcting for slight volume changes accompanying the mixing process. B. APPLICATIONS 1. Model Systems What do volume changes mean in terms of chemical events? The fact that AV is an obligatory quantity ...
... zero time more accurately by correcting for slight volume changes accompanying the mixing process. B. APPLICATIONS 1. Model Systems What do volume changes mean in terms of chemical events? The fact that AV is an obligatory quantity ...
Page 29
... zero. The quantity AVn may be applied directly from the second experiment to the definition of AVm, provided that the amount of pure water in the one limb corresponds precisely to that in the limb containing protein plus water in ...
... zero. The quantity AVn may be applied directly from the second experiment to the definition of AVm, provided that the amount of pure water in the one limb corresponds precisely to that in the limb containing protein plus water in ...
Page 32
... zero for proteins] IV. Density A. METHODS The densities of protein solutions should be known to about 10'5 gm/ml to be useful for most of the purposes described in this chapter. To achieve such accuracy, the sensitivity of the ...
... zero for proteins] IV. Density A. METHODS The densities of protein solutions should be known to about 10'5 gm/ml to be useful for most of the purposes described in this chapter. To achieve such accuracy, the sensitivity of the ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero